Plasmid

Part:BBa_M36161:Design

Designed by: Matt Kandath and Eric Kofman   Group: Stanford BIOE44 - S11   (2012-06-04)

T7 17.5 Plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the possible toxic nature of the gene (as it is proposed to cause cell lysis), a low copy number origin of replication was used in order to somewhat control against over expression of our gene.

Source

Link for T7 genome: http://www.ncbi.nlm.nih.gov/nuccore/NC_001604.1?report=genbank&from=36344&to=36547

References